Gene Knockout: gRNA Design to Analysis

Beginner Bioinformatics - Learn how to design and analyze CRISPR experiments hands-on

Course curriculum

1. Introduction to Gene KO
  FREE PREVIEW
1. Our Hypothesis
  FREE PREVIEW
1. Intro to Cas9
  FREE PREVIEW
2. Intro to DNA repair
  FREE PREVIEW
1. Source & Annotate CCR5 DNA sequence
  FREE PREVIEW
2. Design CRISPR/Cas9 gRNAs targeted to CCR5
3. Introduction to PCR
4. Design PCR primers to be used in analysis
1. How to perform a CRISPR experiment
1. Genetic Analysis Techniques
2. How to analyze a CRISPR experiment
1. Analyze your CCR5 gene editing experiment
2. Gene KO: gRNA Design to Analysis Final Test
1. Final Reminders
2. Gene KO: gRNA Design to Analysis Feedback Survey

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